Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Rhizobium etli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Rhizobium etli |
Protein Variants | Comment | Organism |
---|---|---|
H216N | no differences to quarterny structures compared to wild-type. Mutation results in a 9fold increase in the Km for MgATP in its steady-state cleavage in the absence of pyruvate and a 3fold increase in the Km for MgADP in its steady-state phosphorylation by carbamoyl phosphate. kcat/Km (MgATP)98% decreased compared to wild-type. For MgADP phosphorylation kcat/Km is 99.5% decreased compared to wild-type. The Kd of the enzyme MgATP complex is essentially the same in the wildtype enzyme and H216N but the first-order rate constant for MgATP cleavage in the single-turnover experiments in H216N is only 0.75% of that for the wild-type enzyme, and thus, the MgATP cleavage step is rate-limiting in the steady state for H216N but not for the wild-type enzyme | Rhizobium etli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
ADP | wild-type, MgADP phosphorylation, pH 7.8, 30°C | Rhizobium etli | |
0.008 | - |
ADP | mutant H216N, MgADP phosphorylation, pH 7.8, 30°C | Rhizobium etli | |
0.009 | - |
ATP | wild-type, MgATP cleavage, pH 7.8, 30°C | Rhizobium etli | |
0.082 | - |
ATP | mutant H216N, MgATP cleavage, pH 7.8, 30°C | Rhizobium etli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rhizobium etli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium etli | Q2K340 | - |
- |
Rhizobium etli CFN 42 | Q2K340 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Rhizobium etli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + HCO3- | - |
Rhizobium etli | ADP + phosphate + oxaloacetate | - |
r | |
ATP + pyruvate + HCO3- | - |
Rhizobium etli CFN 42 | ADP + phosphate + oxaloacetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Rhizobium etli |
Synonyms | Comment | Organism |
---|---|---|
RePC | - |
Rhizobium etli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhizobium etli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
pyruvate | co-substrate: HCO3-, mutant H216N, pyruvate carboxylation, pH 7.8, 30°C | Rhizobium etli | |
0.1 | - |
oxaloacetate | co-substrate: ADP + phosphate, mutant H216N, oxaloacetate decarboxylation, pH 7.8, 30°C | Rhizobium etli | |
0.28 | - |
oxaloacetate | co-substrate: ADP + phosphate, wild-type, oxaloacetate decarboxylation, pH 7.8, 30°C | Rhizobium etli | |
11 | - |
pyruvate | co-substrate: HCO3-, wild-type, pyruvate carboxylation, pH 7.8, 30°C | Rhizobium etli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Rhizobium etli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | - |
Rhizobium etli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | mutant H216N, MgATP cleavage, pH 7.8, 30°C | Rhizobium etli | |
2.7 | - |
ADP | mutant H216N, MgADP phosphorylation, pH 7.8, 30°C | Rhizobium etli | |
6.86 | - |
ATP | wild-type, MgATP cleavage, pH 7.8, 30°C | Rhizobium etli | |
589 | - |
ADP | wild-type, MgADP phosphorylation, pH 7.8, 30°C | Rhizobium etli |